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Effects of enucleation method on

Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 61-65 doi: 10.15302/J-FASE-2018227

Abstract: crucial procedure for mammalian somatic cell nuclear transfer (SCNT), especially for domestic animal cloninghigh enucleation rate but has minimal negative effects on the reprogramming potential of oocyte for cloningIn this study, we compared the pig cloning efficiency resulting from blind aspiration-based (BA-B) enucleationand spindle imaging system-assisted (SIS-A) enucleation, and compared the pig SCNT success rate associatedThese data indicate that SIS-A and BAPPS-B enucleation are better approaches for pig SCNT than BA-B enucleation

Keywords： cloning     enucleation     pig     SCNT    

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EFFICIENT CONTAMINANT REMOVAL FROM LIQUID DIGESTATE OF PIG MANURE BY CHEMICAL PRECIPITATION AND CO MINERALIZATION

Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 3,   Pages 479-491 doi: 10.15302/J-FASE-2023480

Abstract:

● LFD was treated by fly ash-based chemical precipitation and CO2 mineralization.

Keywords： anaerobic digestion     chemical oxygen demand     fly ash     ion removal     total phosphate    

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EFFECT OF EXOGENOUS ADDITIVES ON HEAVY METAL PASSIVATION AND NITROGEN RETENTION IN PIG MANURE COMPOSTING

Frontiers of Agricultural Science and Engineering doi: 10.15302/J-FASE-2023487

Abstract:

● Research on heavy metal passivation and nitrogen emissions is necessary for the pig

Keywords： additives     composting     heavy metals passivation     nitrogen retention     pig manure    

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Factors influencing the somatic cell nuclear transfer efficiency in pigs

Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 73-80 doi: 10.15302/J-FASE-2018220

Abstract:

Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiencyconfluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall cloningof blastocysts transferred by somatic cell nuclear transfer (SCNT) did not significantly affect the cloninghighest pregnancy rate, delivery rate and the largest litter size were obtained when Bama Miniature pig

Keywords： blastocyst     donor cell     estrus synchronization     pregnancy rate     pig cloning     somatic cell nuclear transfer    

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AMMONIA DISPERSION FROM MULTI-FLOOR VERSUS STANDARD SINGLE-FLOOR PIG PRODUCTION FACILITIES BASED ON COMPUTATIONAL

Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 3,   Pages 374-389 doi: 10.15302/J-FASE-2023501

Abstract:

● NH3 dispersion from a multi-floor pig building was compared

Keywords： pig building     computational fluid dynamics     ammonia     dispersion    

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Measurement of trimethylamine concentration and evaluation of pig meat natural quality by a spectrophotometric

Mohammod Abdul HAMID,Xi WANG,Xiangdong DING,Chuduan WANG,Xingbo ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 236-241 doi: 10.15302/J-FASE-2014034

Abstract: Pig meat off-flavor is attributed to trimethylamine (TMA) concentration, and it is considered as theIn this study, TMA concentrations in pig meat were determined, and the interactions with breed and genderIn addition, the TMA threshold for meat off-flavor and pig meat natural quality was measured in relationto meat storage and movement, and the influential factors including the pig breed and storage time weremeat breeding programs, and storage time is important for pig meat natural quality.

Keywords： pig meat     trimethylamine (TMA)     influential factor    

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Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review

Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 28-32 doi: 10.15302/J-FASE-2018222

Abstract:

Primordial germ cells (PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by first dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells (iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established. Here, we discuss recent advances in the stem cell field for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.

Keywords： pig     pluripotent stem cells     primordial germ cells     reprogramming    

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Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Strategic Study of CAE 2000, Volume 2, Issue 11,   Pages 1-11

Abstract: neurological deafness gene, was identified using a novel strategy of “Gene Family-Candidate Disease Gene Cloning

Keywords： genetic disease     gene mapping and cloning     gene familiy-candidate disease gene cloning     genome wide scan    

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Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 91-95 doi: 10.15302/J-FASE-2014005

Abstract: good predictor of carcass lean content, an economically important trait, and a main breeding target in piggenomic regions associated with the tenth rib backfat thickness trait were identified in two independent pig

Keywords： backfat thickness     SNP chip     genome-wide association study     compressed mixed linear model     pig    

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Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 307-313 doi: 10.15302/J-FASE-2014039

Abstract: total of 38405 SNPs in 498 animals from 36 diverse breeds were used to detect genomic signatures for pigSeven diversifying signatures between Yucatan hairless pig and hairy pigs were identified on pig chromosomes

Keywords： hairlessness     pig     selective sweeping    

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Factors affecting early embryonic development in cattle: relevance for bovine cloning

Yanna DANG, Kun ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 33-41 doi: 10.15302/J-FASE-2018228

Abstract: Incomplete epigenetic reprogramming is believed to be a major limitation to cloning efficiency.., Trichostatin A) could greatly improve cloning efficiency in both mice and cattle.

Keywords： bovine cloning     embryo development     somatic cell nuclear transfer     X-inactive specific transcript    

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Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 4, doi: 10.1007/s11783-020-1246-9

Abstract: Abstract • Pig feces is the predominant excrement produced by animal husbandry in China. • The PF, Pig-1-BacTaqMan, and Pig-2-BacTaqMan MST assays showed better performance. • The pig-specific MST assays, Pig-Bac2SYBR, Pig-1-BacTaqMan, and Pig-2-BacTaqMan, which are based on Bacteroidales 16S rRNA geneHowever, the two SYBR green qPCR assays (Pig-Bac1SYBR and Pig-Bac2SYBR) cross-reacted with most non-pigUsing the PF and Pig-2-BacTaqMan assays, a field test comparing the levels of pig fecal pollution in

Keywords： Microbial source tracking     Pig fecal pollution     16S rRNA gene markers     Pig-specific Bacteroidales    

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Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was

Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 50-60

Abstract:

The full length cDNA of LH has been cloned from pituitary of half smooth tongue sole C. Günther by using RACE technology for the first time. The cDNA of LH is 670 bp，and the open reading frame is 477 bp encoding peptides of 158 amino acid. Multiple amino acid sequences alignments were performed among vertebrates，and the results showed that tongue sole LH mature peptide was the most similar to their orthologs of Pleuronectiformes and Perciformes which was 58 %～68 % identity. And 12 conserved cysteine residues and 1 N-linked glycosylation site were found in the deduced amino acid sequence. Real-time PCR analysis showed that LH mRNA was expressed in all twelve tissues including brain，pituitary，ovary and so on. The highest expression level was in pituitary. And the stronger amplification signals were detected in the brain and gonad than all other non- pituitary tissues. It suggested that LH may had the more physiological functions because LH mRNA of tongue sole expressed widely in non-pituitary tissues. Quantitative real- time PCR was used for analyzing the expression of female C. semilaevis LH mRNA in the reproductive cycle. The results showed that LH mRNA was expressed in all three tissues including brain，pituitary and ovary from the stage Ⅱto stage Ⅵ， and the highest expression level appeared on the stage Ⅳ and stage Ⅴwhich suggested that the main function of LH is to promote eggs finally mature and discharge. Radio immunoassay （RIA）marked by 125I was used to detect the concentration of plasma LH in C.semilaevis. The results showed that the biggest plasma LH concentration appareed on the stage of Ⅴwhich also suggested that the main function of LH was to promote eggs finally mature and discharge.

Keywords： Cynoglossus semilaevis Günther     LH     cDNA cloning     Quantitative real-time PCR     RIA    

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First Pig-to-Live Human Xenotransplant Produces Mixed Results

Sarah C.P. Williams

Engineering 2022, Volume 16, Issue 9,   Pages 6-8 doi: 10.1016/j.eng.2022.07.006

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Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning Article

Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao

Engineering 2023, Volume 24, Issue 5,   Pages 73-83 doi: 10.1016/j.eng.2022.04.016

Abstract:

Since the first cloned sheep was produced in 1996, cloning has attracted considerable attention becauseThis low success rate is considered to be the major limitation in the extensive application of cloningWe then developed a robotic batch SCNT system to apply this micromanipulation method to animal cloningThe success rate of cloning was significantly increased compared to that of traditional methods (2.5%In addition to the cloning technique, the intracellular strain evaluation-based enucleation method is

Keywords： Oocyte enucleation     Robotic cloning     Intracellular strain     Intracellular damage     Optical flow    

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Title Author Date Type Operation

Effects of enucleation method on

Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI

Journal Article

EFFICIENT CONTAMINANT REMOVAL FROM LIQUID DIGESTATE OF PIG MANURE BY CHEMICAL PRECIPITATION AND CO MINERALIZATION

Journal Article

EFFECT OF EXOGENOUS ADDITIVES ON HEAVY METAL PASSIVATION AND NITROGEN RETENTION IN PIG MANURE COMPOSTING

Journal Article

Factors influencing the somatic cell nuclear transfer efficiency in pigs

Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI

Journal Article

AMMONIA DISPERSION FROM MULTI-FLOOR VERSUS STANDARD SINGLE-FLOOR PIG PRODUCTION FACILITIES BASED ON COMPUTATIONAL

Journal Article

Measurement of trimethylamine concentration and evaluation of pig meat natural quality by a spectrophotometric

Mohammod Abdul HAMID,Xi WANG,Xiangdong DING,Chuduan WANG,Xingbo ZHAO

Journal Article

Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review

Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA

Journal Article

Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Journal Article

Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

Journal Article

Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

Journal Article

Factors affecting early embryonic development in cattle: relevance for bovine cloning

Yanna DANG, Kun ZHANG

Journal Article

Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Journal Article

Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was

Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao

Journal Article

First Pig-to-Live Human Xenotransplant Produces Mixed Results

Sarah C.P. Williams

Journal Article

Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning

Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao

Journal Article